Solution:
Root Cause: Missing NTFS Permissions on Database and Log Directories Exchange databases require strict permission inheritance. If permissions on the database
.edb folder or transaction log directory are modified, the Exchange Trusted Subsystem or SYSTEM accounts lose access, preventing the Information Store from opening and mounting the database.
Step-by-Step Fix: 1 Grant Permissions via PowerShell or File Properties:
Open PowerShell as Administrator and grant full control to the Exchange Trusted Subsystem: Copy
powershell
$Acl = Get-Acl "E:\Mailbox\Mailbox Database"
$Permission = "Exchange Trusted Subsystem", "FullControl", "ContainerInherit,ObjectInherit", "None", "Allow"
$Acl.AddAccessRule($Permission)
Set-Acl "E:\Mailbox\Mailbox Database" $Acl
2 Verify SYSTEM and Administrators Access:
Ensure NT AUTHORITY\SYSTEM and local Administrators also retain Full Control permissions on both database and log folders.